Journal Article

PreJuSER-19648

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Systems Analysis of a Maize Leaf Developmental Gradient Redefines the Current C4 Model and Provides Canditates for Regulation

Pick, T. R. ; Bräutigam, A.FZJ* ; Schlüter, U. ; Denton, A. K. ; Colmsee, C. ; Scholz, U. ; Fahnenstich, H. ; Pieruschka, R.FZJ* ; Rascher, U.FZJ* ; Sonnewald, U. ; Weber, A. P. M.FZJ*

2012
Soc. Rockville, Md.

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Please use a persistent id in citations: doi:10.1105/tpc.111.090324

Abstract: We systematically analyzed a developmental gradient of the third maize (Zea mays) leaf from the point of emergence into the light to the tip in 10 continuous leaf slices to study organ development and physiological and biochemical functions. Transcriptome analysis, oxygen sensitivity of photosynthesis, and photosynthetic rate measurements showed that the maize leaf undergoes a sink-to-source transition without an intermediate phase of C(3) photosynthesis or operation of a photorespiratory carbon pump. Metabolome and transcriptome analysis, chlorophyll and protein measurements, as well as dry weight determination, showed continuous gradients for all analyzed items. The absence of binary on-off switches and regulons pointed to a morphogradient along the leaf as the determining factor of developmental stage. Analysis of transcription factors for differential expression along the leaf gradient defined a list of putative regulators orchestrating the sink-to-source transition and establishment of C(4) photosynthesis. Finally, transcriptome and metabolome analysis, as well as enzyme activity measurements, and absolute quantification of selected metabolites revised the current model of maize C(4) photosynthesis. All data sets are included within the publication to serve as a resource for maize leaf systems biology.

Keyword(s): Chlorophyll: analysis (MeSH) ; Chlorophyll: chemistry (MeSH) ; Cluster Analysis (MeSH) ; Enzyme Activation (MeSH) ; Gene Expression Regulation, Plant (MeSH) ; Genes, Plant (MeSH) ; Light (MeSH) ; Malates: chemistry (MeSH) ; Metabolome (MeSH) ; Oxygen: chemistry (MeSH) ; Photosynthesis (MeSH) ; Plant Leaves: chemistry (MeSH) ; Plant Leaves: genetics (MeSH) ; Plant Leaves: physiology (MeSH) ; Plant Proteins: chemistry (MeSH) ; Plant Proteins: genetics (MeSH) ; Pyruvic Acid: chemistry (MeSH) ; Transcription Factors: chemistry (MeSH) ; Transcription Factors: genetics (MeSH) ; Transcription, Genetic (MeSH) ; Transcriptome (MeSH) ; Zea mays: chemistry (MeSH) ; Zea mays: genetics (MeSH) ; Zea mays: physiology (MeSH) ; Malates ; Plant Proteins ; Transcription Factors ; Pyruvic Acid ; Chlorophyll ; malic acid ; Oxygen ; J

Note: This study was supported by a grant of the German Federal Ministry of Education and Research (BioEnergy 2021, OPTIMAS) and grants from the German Research Foundation (IRTG 1525 and FOR 1186 PROMICS to A.P.M.W.). We thank Katrin L. Weber for support with GC-MS analyses.

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Contributing Institute(s):

1. Pflanzenwissenschaften (IBG-2)

Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2012

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Database coverage:
; BIOSIS Previews ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; National-Konsortium ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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