Journal Article

PreJuSER-29267

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Surface anchoring reduces the lifetime of single specific bonds

Nguyen-Duong, C. J. ; Koch, K.-W. ; Merkel, R.FZJ*

2003
EDP Sciences Les Ulis

This record in other databases:  

Please use a persistent id in citations: doi:10.1209/epl/i2003-00311-6

Abstract: We investigated the force-induced dissociation of single specific bonds between protein A and immunoglobulin G. In these experiments both proteins were tethered to microbeads. Piconewton forces were applied by means of an ultrasoft spring. Upon bond formation the thermal fluctuations of this soft spring were reduced. This resulted in a sizeable contribution to the free enthalpy of bonding. Including this effect we calculated the relation between the lifetime of bonds connecting both microbeads and the lifetime of the bond between free molecules. We found that in the first case the lifetime depended on the stiffness of the force probe. Moreover, the lifetime was reduced by almost two orders of magnitude. Both predictions were verified in our experiments.

Keyword(s): J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Institut für biologisch-anorganische Grenzflächen (ISG-4)
2. Zelluläre Signalverarbeitung (IBI-1)

Research Program(s):

1. Neurowissenschaften (L01)
2. Kondensierte Materie (M02)

Appears in the scientific report 2003

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Database coverage:
Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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