Journal Article

PreJuSER-47465

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Two-component systems of Corynebacterium glutamicum: deletion analysis and involvement of the PhoS-PhoR-system in the phosphate starvation response

Kocan, M.FZJ* ; Schaffer, S.FZJ* ; Ishige, T.FZJ* ; Sorger-Herrmann, U.FZJ* ; Wendisch, V. F.FZJ* ; Bott, M.FZJ*

2006
Soc. Washington, DC

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Please use a persistent id in citations: http://hdl.handle.net/2128/2425  doi:10.1128/JB.188.2.724-732.2006

Abstract: Corynebacterium glutamicum contains genes for 13 two-component signal transduction systems. In order to test for their essentiality and involvement in the adaptive response to phosphate (Pi) starvation, a set of 12 deletion mutants was constructed. One of the mutants was specifically impaired in its ability to grow under Pi limitation, and therefore the genes lacking in this strain were named phoS (encoding the sensor kinase) and phoR (encoding the response regulator). DNA microarray analyses with the C. glutamicum wild type and the DeltaphoRS mutant supported a role for the PhoRS system in the adaptation to Pi starvation. In contrast to the wild type, the DeltaphoRS mutant did not induce the known Pi starvation-inducible (psi) genes within 1 hour after a shift from Pi excess to Pi limitation, except for the pstSCAB operon, which was still partially induced. This indicates an activator function for PhoR and the existence of at least one additional regulator of the pst operon. Primer extension analysis of selected psi genes (pstS, ugpA, phoR, ushA, and nucH) confirmed the microarray data and provided evidence for positive autoregulation of the phoRS genes.

Keyword(s): Bacterial Proteins: genetics (MeSH) ; Bacterial Proteins: metabolism (MeSH) ; Base Sequence (MeSH) ; Corynebacterium glutamicum: genetics (MeSH) ; Corynebacterium glutamicum: growth & development (MeSH) ; Corynebacterium glutamicum: metabolism (MeSH) ; Culture Media (MeSH) ; Gene Deletion (MeSH) ; Gene Expression Regulation, Bacterial (MeSH) ; Genes, Bacterial (MeSH) ; Molecular Sequence Data (MeSH) ; Operon (MeSH) ; Phosphates (MeSH) ; Protein Kinases: genetics (MeSH) ; Protein Kinases: metabolism (MeSH) ; RNA, Messenger: genetics (MeSH) ; Signal Transduction (MeSH) ; Bacterial Proteins ; Culture Media ; Phosphates ; RNA, Messenger ; Protein Kinases ; J

Note: Record converted from VDB: 12.11.2012

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Contributing Institute(s):

1. Biotechnologie 1 (IBT-1)

Research Program(s):

1. Biotechnologie (PBT)

Appears in the scientific report 2006

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Database coverage:

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