Journal Article

PreJuSER-6389

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Simultaneous phenotyping of leaf growth and chlorophyll fluorescence via GROWSCREEN FLUORO allows detection of stress tolerance in Arabidopsis thaliana and other rosette plants

Jansen, M.FZJ* ; Gilmer, F.FZJ* ; Biskup, B.FZJ* ; Nagel, K. A.FZJ* ; Rascher, U.FZJ* ; Fischbach, A.FZJ* ; Briem, S.FZJ* ; Dreissen, G.FZJ* ; Tittmann, S.FZJ* ; Braun, S.FZJ* ; De Jaeger, I. ; Metzlaff, M. ; Schurr, U.FZJ* ; Scharr, H.FZJ* ; Walter, A.FZJ*

2009
CSIRO Publ. Collingwood, Victoria

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Please use a persistent id in citations: doi:10.1071/FP09095

Abstract: Stress caused by environmental factors evokes dynamic changes in plant phenotypes. In this study, we deciphered simultaneously the reaction of plant growth and chlorophyll fluorescence related parameters using a novel approach which combines existing imaging technologies (GROWSCREEN FLUORO). Three different abiotic stress situations were investigated demonstrating the benefit of this approach to distinguish between effects related to (1) growth, (2) chlorophyll-fluorescence, or (3) both of these aspects of the phenotype. In a drought stress experiment with more than 500 plants, poly(ADP-ribose) polymerase (PARP) deficient lines of Arabidopsis thaliana (L.) Heynh showed increased relative growth rates (RGR) compared with C24 wild-type plants. In chilling stress, growth of PARP and C24 lines decreased rapidly, followed by a decrease in F-v/F-m. Here, PARP-plants showed a more pronounced decrease of F-v/F-m than C24, which can be interpreted as a more efficient strategy for survival in mild chilling stress. Finally, the reaction of Nicotiana tabacum L. to altered spectral composition of the intercepted light was monitored as an example of a moderate stress situation that affects chlorophyll-fluorescence related, but not growth-related parameters. The examples investigated in this study show the capacity for improved plant phenotyping based on an automated and simultaneous evaluation of growth and photosynthesis at high throughput.

Keyword(s): J ; chilling stress (auto) ; drought (auto) ; dynamic processes (auto) ; image processing (auto) ; Nicotiana tabacum (auto) ; PARP (auto) ; phenomics (auto)

Note: B. Biskup and S. Tittmann acknowledge support of their PhD theses by the Heinrich-Heine University of Dusseldorf, Germany. We are grateful to I.-L. Lai and R. Poire for testing earlier versions of the setup in preliminary experiments and to B. Uhlig, M. Schmitz and B. Greve for assisting in plant cultivation. We thank K. H. Kjaer and S. Matsubara for constructive comments on earlier versions of this manuscript.

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Research Program(s):

1. Terrestrische Umwelt (P24)

Appears in the scientific report 2009

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