Journal Article

FZJ-2017-01983

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Uptake dynamics of graphene quantum dots into primary human blood cells following in vitro exposure

Fasbender, S. ; Allani, S. ; Wimmenauer, C. ; Cadeddu, R.-P. ; Raba, K. ; Fischer, J. C. ; Bulat, B. ; Luysberg, M.FZJ* ; Seidel, C. A.  M. ; Heinzel, T. ; Haas, R. (Corresponding author)

2017
RSC Publishing London

This record in other databases:    

Please use a persistent id in citations: http://hdl.handle.net/2128/13898  doi:10.1039/C6RA27829A

Abstract: Human leukocytes obtained from samples of leukapheresis products of three healthy donors stimulated by granulocyte colony stimulating factor (G-CSF) were exposed to graphene quantum dots. A time- and concentration dependent uptake was observed with a significantly greater uptake into monocytes and granulocytes in comparison to lymphocytes, suggesting a better incorporation ability of cells with phagocytotic properties. The uptake rates also correlate with the cell membrane area. Looking at the different lymphoid subsets a greater uptake was found into CD19+ B-, CD56+ natural killer cells and CD34+ hematopoietic stem cells (HSC) in comparison to CD4+ T- and CD8+ T cells. Independent of the cell type studied, the observed uptake dynamics is consistent with a diffusion-driven process, which allows the determination of cell-specific membrane permeabilities for the graphene quantum dots. The toxicity of the quantum dots is relatively low resulting in a 90% viability of the entire leukocyte population after 36 hours of exposure to GQDs at a concentration of 500 μg ml−1.

---

Contributing Institute(s):

1. Physik Nanoskaliger Systeme (ER-C-1)

Research Program(s):

1. 143 - Controlling Configuration-Based Phenomena (POF3-143) (POF3-143)

Appears in the scientific report 2017

---

Database coverage:
; ; ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---