Early stages of clathrin aggregation at a membrane in coarse-grained simulations

Giani, M.; Briels, Willem; den Otter, W. K.

doi:10.1063/1.4979985

Journal Article

FZJ-2017-03611

Early stages of clathrin aggregation at a membrane in coarse-grained simulations

Giani, M. ; den Otter, W. K. (Corresponding author) ; Briels, W.FZJ*

2017
American Institute of Physics Melville, NY

The journal of chemical physics 146(15), 155102 - (2017) [10.1063/1.4979985]

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Please use a persistent id in citations: http://hdl.handle.net/2128/18955 doi:10.1063/1.4979985

Abstract: The self-assembly process of clathrin coated pits during endocytosis has been simulated by combining and extending coarse grained models of the clathrin triskelion, the adaptor protein AP2, and a flexible network membrane. The AP2’s core, upon binding to membrane and cargo, releases a motif that can bind clathrin. In conditions where the core-membrane-cargo binding is weak, the binding of this motif to clathrin can result in a stable complex. We characterize the conditions and mechanisms resulting in the formation of clathrin lattices that curve the membrane, i.e., clathrin coated pits. The mechanical properties of the AP2 β linker appear crucial to the orientation of the curved clathrin lattice relative to the membrane, with wild-type short linkers giving rise to the inward curving buds enabling endocytosis while long linkers produce upside-down cages and outward curving bulges

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