Journal Article

FZJ-2018-06000

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Identification of stable and enantioselective lipases for biotechnological applications

Jaeger, K.-E.FZJ* ; Fulton, A.FZJ* ; Schwaneberg, U. ; Pietruszka, J.FZJ* ; Kovacic, F. (Corresponding author)FZJ*

2018
Zagreb

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Please use a persistent id in citations: http://hdl.handle.net/2128/19862  doi:10.5562/cca3357

Abstract: The implementation of biocatalytic reactions in biotechnological processes requires enzymes with suitable properties. Thus, optimization of existing or identification of novel enzymes is needed. For enzyme optimization, various mutagenesis approaches are used yielding libraries with a large number of protein variants. Additionally, novel enzymes can be identified from metagenomic libraries, which contain bacterial clones expressing DNA isolated from different habitats. Activity-based screening of such libraries is needed to identify enzyme candidates of interest. Here, we provide a protocol for screening of regio- and enantioselective lipases stable under industrially relevant conditions.

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Contributing Institute(s):

1. Institut für Molekulare Enzymtechnologie (HHUD) (IMET)
2. Biotechnologie (IBG-1)
3. Institut für Bioorganische Chemie (HHUD) (IBOC)

Research Program(s):

1. 581 - Biotechnology (POF3-581) (POF3-581)

Appears in the scientific report 2018

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Database coverage:
; ; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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