Journal Article

FZJ-2019-01609

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Anoctamin-4 is a bona fide Ca2+-dependent non-selective cation channel

Reichhart, N. ; Schöberl, S. ; Keckeis, S. ; Alfaar, A. S. ; Roubeix, C. ; Cordes, M. ; Crespo-Garcia, S. ; Haeckel, A. ; Kociok, N. ; Föckler, R. ; Fels, G. ; Mataruga, A.FZJ* ; Rauh, R. ; Milenkovic, V. M. ; Zühlke, K. ; Klussmann, E. ; Schellenberger, E. ; Strauß, O. (Corresponding author)

2019
Macmillan Publishers Limited, part of Springer Nature [London]

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/21709  doi:10.1038/s41598-018-37287-y

Abstract: Changes in cell function occur by specific patterns of intracellular Ca2+, activating Ca2+-sensitive proteins. The anoctamin (TMEM16) protein family has Ca2+-dependent ion channel activity, which provides transmembrane ion transport, and/or Ca2+-dependent phosphatidyl-scramblase activity. Using amino acid sequence analysis combined with measurements of ion channel function, we clarified the so far unknown Ano4 function as Ca2+-dependent, non-selective monovalent cation channel; heterologous Ano4 expression in HEK293 cells elicits Ca2+ activated conductance with weak selectivity of K+ > Na+ > Li+. Endogenously expressed Ca2+-dependent cation channels in the retinal pigment epithelium were identified as Ano4 by KO mouse-derived primary RPE cells and siRNA against Ano4. Exchanging a negatively charged amino acid in the putative pore region (AA702–855) into a positive one (E775K) turns Ano4-elicited currents into Cl− currents evidencing its importance for ion selectivity. The molecular identification of Ano4 as a Ca2+-activated cation channel advances the understanding of its role in Ca2+ signaling.

---

Contributing Institute(s):

1. Zelluläre Biophysik (ICS-4)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2019

---

Database coverage:
; ; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

---