Journal Article

FZJ-2019-05978

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Unusual features of the c-ring of F1FO ATP synthases

Vlasov, A. V. ; Kovalev, K. V. ; Marx, S.-H. ; Round, E. S. ; Gushchin, I. Y. ; Polovinkin, V. A. ; Tsoy, N. M. ; Okhrimenko, I. S. ; Borshchevskiy, V. I.FZJ* ; Büldt, G. D. ; Ryzhykau, Y. L. ; Rogachev, A. V. ; Chupin, V. V. ; Kuklin, A. I. ; Dencher, N. A. ; Gordeliy, V. I. (Corresponding author)FZJ*

2019
Macmillan Publishers Limited, part of Springer Nature [London]

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Please use a persistent id in citations: http://hdl.handle.net/2128/23897  doi:10.1038/s41598-019-55092-z

Abstract: Membrane integral ATP synthases produce adenosine triphosphate, the universal “energy currency” of most organisms. However, important details of proton driven energy conversion are still unknown. We present the first high-resolution structure (2.3 Å) of the in meso crystallized c-ring of 14 subunits from spinach chloroplasts. The structure reveals molecular mechanisms of intersubunit contacts in the c14-ring, and it shows additional electron densities inside the c-ring which form circles parallel to the membrane plane. Similar densities were found in all known high-resolution structures of c-rings of F1FO ATP synthases from archaea and bacteria to eukaryotes. The densities might originate from isoprenoid quinones (such as coenzyme Q in mitochondria and plastoquinone in chloroplasts) that is consistent with differential UV-Vis spectroscopy of the c-ring samples, unusually large distance between polar/apolar interfaces inside the c-ring and universality among different species. Although additional experiments are required to verify this hypothesis, coenzyme Q and its analogues known as electron carriers of bioenergetic chains may be universal cofactors of ATP synthases, stabilizing c-ring and prevent ion leakage through it.

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Contributing Institute(s):

1. Strukturbiochemie (ICS-6)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2019

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