Journal Article

FZJ-2021-01004

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Heterologous Production of β-Caryophyllene and Evaluation of Its Activity against Plant Pathogenic Fungi

Hilgers, F.FZJ* ; Habash, S. S. ; Loeschcke, A.FZJ* ; Ackermann, Y.FZJ* ; Neumann, S. ; Heck, A.FZJ* ; Klaus, O.FZJ* ; Hage-Hülsmann, J.FZJ* ; Grundler, F. M. W. ; Jaeger, K.-E.FZJ* ; Sylvia S. Schleker, A. (Corresponding author) ; Drepper, T. (Corresponding author)FZJ*

2021
MDPI Basel

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Please use a persistent id in citations: http://hdl.handle.net/2128/27599  doi:10.3390/microorganisms9010168

Abstract: Terpenoids constitute one of the largest and most diverse groups within the class of secondary metabolites, comprising over 80,000 compounds. They not only exhibit important functions in plant physiology but also have commercial potential in the biotechnological, pharmaceutical, and agricultural sectors due to their promising properties, including various bioactivities against pathogens, inflammations, and cancer. In this work, we therefore aimed to implement the plant sesquiterpenoid pathway leading to β-caryophyllene in the heterologous host Rhodobacter capsulatus and achieved a maximum production of 139 ± 31 mg L−1 culture. As this sesquiterpene offers various beneficial anti-phytopathogenic activities, we evaluated the bioactivity of β-caryophyllene and its oxygenated derivative β-caryophyllene oxide against different phytopathogenic fungi. Here, both compounds significantly inhibited the growth of Sclerotinia sclerotiorum and Fusarium oxysporum by up to 40%, while growth of Alternaria brassicicola was only slightly affected, and Phoma lingam and Rhizoctonia solani were unaffected. At the same time, the compounds showed a promising low inhibitory profile for a variety of plant growth-promoting bacteria at suitable compound concentrations. Our observations thus give a first indication that β-caryophyllene and β-caryophyllene oxide are promising natural agents, which might be applicable for the management of certain plant pathogenic fungi in agricultural crop production.

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Contributing Institute(s):

1. Institut für Molekulare Enzymtechnologie (HHUD) (IMET)
2. Biotechnologie (IBG-1)

Research Program(s):

1. 217 - Für eine nachhaltige Bio-Ökonomie – von Ressourcen zu Produkten (POF4-217) (POF4-217)
2. 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) (POF4-217)

Appears in the scientific report 2021

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