Journal Article

FZJ-2021-02566

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Robotic integration enables autonomous operation of laboratory scale stirred tank bioreactors with model‐driven process analysis

Morschett, H. ; Tenhaef, N.FZJ* ; Hemmerich, J. ; Herbst, L.FZJ* ; Spiertz, M. ; Dogan, D.FZJ* ; Wiechert, W.FZJ* ; Noack, S.FZJ* ; Oldiges, M. (Corresponding author)FZJ*

2021
Wiley New York, NY [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/29679  doi:10.1002/bit.27795

Abstract: Given its geometric similarity to large-scale production plants and the excellent possibilities for precise process control and monitoring, the classic stirred tank bioreactor (STR) still represents the gold standard for bioprocess development at a laboratory scale. However, compared to microbioreactor technologies, bioreactors often suffer from a low degree of process automation and deriving key performance indicators (KPIs) such as specific rates or yields often requires manual sampling and sample processing. A widely used parallelized STR setup was automated by connecting it to a liquid handling system and controlling it with a custom-made process control system. This allowed for the setup of a flexible modular platform enabling autonomous operation of the bioreactors without any operator present. Multiple unit operations like automated inoculation, sampling, sample processing and analysis, and decision making, for example for automated induction of protein production were implemented to achieve such functionality. The data gained during application studies was used for fitting of bioprocess models to derive relevant KPIs being in good agreement with literature. By combining the capabilities of STRs with the flexibility of liquid handling systems, this platform technology can be applied to a multitude of different bioprocess development pipelines at laboratory scale.

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Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) (POF4-217)

Appears in the scientific report 2021

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; DEAL Wiley ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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