Journal Article

FZJ-2021-03192

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png PspA adopts an ESCRT-III-like fold and remodels bacterial membranes

Junglas, B. (Corresponding author)FZJ* ; Huber, S. T. ; Heidler, T.FZJ* ; Schlösser, L. ; Mann, D.FZJ* ; Hennig, R. ; Clarke, M.Extern* ; Hellmann, N.Extern* ; Schneider, D. ; Sachse, C. (Corresponding author)FZJ*

2021
Elsevier New York, NY

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Please use a persistent id in citations: http://hdl.handle.net/2128/31853  doi:10.1016/j.cell.2021.05.042

Abstract: PspA is the main effector of the phage shock protein (Psp) system and preserves the bacterial inner membrane integrity and function. Here, we present the 3.6 Å resolution cryoelectron microscopy (cryo-EM) structure of PspA assembled in helical rods. PspA monomers adopt a canonical ESCRT-III fold in an extended open conformation. PspA rods are capable of enclosing lipids and generating positive membrane curvature. Using cryo-EM, we visualized how PspA remodels membrane vesicles into μm-sized structures and how it mediates the formation of internalized vesicular structures. Hotspots of these activities are zones derived from PspA assemblies, serving as lipid transfer platforms and linking previously separated lipid structures. These membrane fusion and fission activities are in line with the described functional properties of bacterial PspA/IM30/LiaH proteins. Our structural and functional analyses reveal that bacterial PspA belongs to the evolutionary ancestry of ESCRT-III proteins involved in membrane remodeling.

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Contributing Institute(s):

1. Strukturbiologie (ER-C-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)
2. 5352 - Understanding the Functionality of Soft Matter and Biomolecular Systems (POF4-535) (POF4-535)

Appears in the scientific report 2021

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Database coverage:
; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 30 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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