Journal Article

FZJ-2022-00285

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Open-Bundle Structure as the Unfolding Intermediate of Cytochrome c′ Revealed by Small Angle Neutron Scattering

Yamaguchi, T. ; Akao, K. ; Koutsioubas, A.FZJ* ; Frielinghaus, H.FZJ* ; Kohzuma, T. (Corresponding author)

2022
MDPI Basel

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Please use a persistent id in citations: http://hdl.handle.net/2128/30010  doi:10.3390/biom12010095

Abstract: The dynamic structure changes, including the unfolding, dimerization, and transition from the compact to the open-bundle unfolding intermediate structure of Cyt c', were detected by a small-angle neutron scattering experiment (SANS). The structure of Cyt c' was changed into an unstructured random coil at pD = 1.7 (Rg = 25 Å for the Cyt c' monomer). The four-α-helix bundle structure of Cyt c' at neutral pH was transitioned to an open-bundle structure (at pD ~13), which is given by a numerical partial scattering function analysis as a joint-clubs model consisting of four clubs (α-helices) connected by short loops. The compactly folded structure of Cyt c' (radius of gyration, Rg = 18 Å for the Cyt c' dimer) at neutral or mildly alkaline pD transited to a remarkably larger open-bundle structure at pD ~13 (Rg = 25 Å for the Cyt c' monomer). The open-bundle structure was also supported by ab initio modeling.

Keyword(s): Polymers, Soft Nano Particles and Proteins (1st) ; Biology (2nd) ; Soft Condensed Matter (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. Neutronenstreuung (JCNS-1)
3. JCNS-4 (JCNS-4)
4. Heinz Maier-Leibnitz Zentrum (MLZ)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ) (POF4-6G4) (POF4-6G4)
2. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)

Experiment(s):

1. KWS-1: Small angle scattering diffractometer (NL3b)

Appears in the scientific report 2022

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Database coverage:
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