Journal Article

FZJ-2023-01740

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Multi-angle in situ dynamic light scattering at a neutron spin echo spectrometer

Vögl, F. ; Balacescu, L. ; Holderer, O.FZJ* ; Pasini, S.FZJ* ; Staringer, S.FZJ* ; Brandl, G.FZJ* ; Ossovyi, V.FZJ* ; Feilbach, H.FZJ* ; Müller-Buschbaum, P. ; Stadler, A. M.FZJ* ; Fitter, J.FZJ* ; Schrader, T. E. (Corresponding author)FZJ*

2023
American Institute of Physics [Erscheinungsort nicht ermittelbar]

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Please use a persistent id in citations: http://hdl.handle.net/2128/34274  doi:10.1063/5.0136367

Abstract: A new sample environment, called Bio-Oven, has been built for the Neutron Spin Echo (NSE) SpectrometerJ-NSE Phoenix. It provides active temperature control and the possibility to perform Dynamic Light Scattering(DLS) measurements during the neutron measurement. DLS provides diffusion coefficients of the dissolvednanoparticles and thus one can monitor the aggregation state of the sample on a time scale of minutes duringthe spin echo measurement times on the order of days. This approach helps to validate the NSE data or toreplace the sample when its aggregation state influences the spin echo measurement results. The new Bio-Ovenis an in situ DLS setup based on optical fibers decoupling the free space optics around the sample cuvettein a lightproof casing from the laser sources and the detectors. It collects light from three scattering anglessimultaneously. Six different values of momentum transfer can be accessed by switching between two differentlaser colors. Test experiments were performed with silica nanoparticles with diameters ranging from 20 nm upto 300 nm. Their hydrodynamic radii were determined from DLS measurements and compared with the onesobtained by a commercial particle sizer. It was demonstrated that also the static light scattering signal can beprocessed and gives meaningful results. The protein sample apomyoglobin was used for a long-term test and ina first neutron measurement using the new Bio-Oven. The results prove that the aggregation state of the samplecan be followed using in situ DLS along with the neutron measurement.

Keyword(s): Instrument and Method Development (1st) ; Biology (2nd) ; Condensed Matter Physics (2nd) ; Instrument and Method Development (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. Neutronenstreuung (JCNS-1)
3. JCNS-4 (JCNS-4)
4. Heinz Maier-Leibnitz Zentrum (MLZ)
5. Strukturbiologie (ER-C-3)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (FZJ) (POF4-6G4) (POF4-6G4)
2. 632 - Materials – Quantum, Complex and Functional Materials (POF4-632) (POF4-632)

Experiment(s):

1. J-NSE: Neutron spin-echo spectrometer (NL2ao)

Appears in the scientific report 2023

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; National-Konsortium ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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