Journal Article

FZJ-2024-02676

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png High-Throughput Correlative Light and Cryo-Electron Microscopy Pipeline Using PRIMO Micropatterning, CERES Ice Shield and the METEOR In-Chamber Fluorescence Light Microscope

Berkamp, S.FZJ* ; Smeets, M. ; Caignard, A. ; Jani, R. ; Daviran, D. ; Sachse, C. (Corresponding author)FZJ*

2023
Oxford University Press Oxford

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Please use a persistent id in citations: doi:10.1093/micmic/ozad067.521  doi:10.34734/FZJ-2024-02676

Abstract: Correlative light and cryo-electron microscopy (CLEM) can be used to study macromolecular structures in vitreous state of the complex environment of the cell. The associated workflow, however, is still cumbersome and low throughput. It includes many steps and is therefore prone to ice contamination, cracked lamellae or devitrification all of which can result in sample loss. We have implemented several technological improvements to the workflow resulting in a much simpler and higher throughput method, yielding more lamellae with reduced ice contamination.

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Contributing Institute(s):

1. Strukturbiologie (ER-C-3)

Research Program(s):

1. 5352 - Understanding the Functionality of Soft Matter and Biomolecular Systems (POF4-535) (POF4-535)
2. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2024

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Database coverage:
; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF < 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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