Hydration dependent studies of highly aligned multilayer lipid membranes by neutron scattering

Trapp, M.; Unruh, T.; Demé, B.; Peters, J.; Gutberlet, T.; Tehei, M.; Juranyi, F.

doi:10.1063/1.3495973

Journal Article

Hydration dependent studies of highly aligned multilayer lipid membranes by neutron scattering

Trapp, M. ; Gutberlet, T.FZJ* ; Juranyi, F. ; Unruh, T. ; Demé, B. ; Tehei, M. ; Peters, J.

2010
American Institute of Physics Melville, NY

The journal of chemical physics 133, 164505 (2010) [10.1063/1.3495973]

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Please use a persistent id in citations: http://hdl.handle.net/2128/19492 doi:10.1063/1.3495973

Abstract: We investigated molecular motions on a picosecond timescale of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) model membranes as a function of hydration by using elastic and quasielastic neutron scattering. Two different hydrations corresponding to approximately nine and twelve water molecules per lipid were studied, the latter being the fully hydrated state. In our study, we focused on head group motions by using chain deuterated lipids. Information on in-plane and out-of-plane motions could be extracted by using solid supported DMPC multilayers. Our studies confirm and complete former investigations by König et al. [J. Phys. II (France) 2, 1589 (1992)] and Rheinstädter et al. [Phys. Rev. Lett. 101, 248106 (2008)] who described the dynamics of lipid membranes, but did not explore the influence of hydration on the head group dynamics as presented here. From the elastic data, a clear shift of the main phase transition from the P(β) ripple phase to the L(α) liquid phase was observed. Decreasing water content moves the transition temperature to higher temperatures. The quasielastic data permit a closer investigation of the different types of head group motion of the two samples. Two different models are needed to fit the elastic incoherent structure factor and corresponding radii were calculated. The presented data show the strong influence hydration has on the head group mobility of DMPC.

Keyword(s): Dimyristoylphosphatidylcholine: chemistry (MeSH) ; Membranes, Artificial (MeSH) ; Neutron Diffraction (MeSH) ; Phase Transition (MeSH) ; Water: chemistry (MeSH) ; Membranes, Artificial ; Dimyristoylphosphatidylcholine ; Water ; J

Classification:

Physics, Atomic, Molecular & Chemical

Note: The authors thank F. Natali and S. Busch for the fruitful discussions. M. Trapp was supported by a Ph.D. scholarship from the French Ministry for Research and Technology. This research project has been supported by the European Commission under the Sixth Framework Program through the Key Action: Strengthening the European Research Area, Research Infrastructures (Contract No. RII3-CT-2003-5059825). We acknowledge the ILL and the FRM II for the allocation of beamtime and the financial support from the Access to Major Research Facilities Program which is a component of the International Science Linkages Program established under the Australian Government's innovation statement, Backing Australia's Ability.

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