Altered Turnover of beta-Carotene and Chl a in Arabidopsis Leaves Treated with Lincomycin or Norflurazon

Beisel, K.G.; Schurr, U.; Matsubara, S.

doi:10.1093/pcp/pcr069

Journal Article

PreJuSER-17572

Altered Turnover of beta-Carotene and Chl a in Arabidopsis Leaves Treated with Lincomycin or Norflurazon

Beisel, K. G.FZJ* ; Schurr, U.FZJ* ; Matsubara, S.FZJ*

2011
Oxford University Press Oxford

Plant & cell physiology 52, 1193 - 1203 (2011) [10.1093/pcp/pcr069]

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Please use a persistent id in citations: doi:10.1093/pcp/pcr069

Abstract: Interactions between β-carotene (β-C) and Chl a turnover were investigated in relation to photoinhibition and D1 protein turnover in mature leaves of Arabidopsis (Arabidopsis thaliana) by ¹⁴CO₂ pulse-chase labeling. Following a 2 h treatment of leaves with water, lincomycin (Linco; an inhibitor of chloroplast protein synthesis) or norflurazon (NF; an inhibitor of carotenoid biosynthesis at phytoene desaturation) in the dark, ¹⁴CO₂ was applied to the leaves for 30 min under control light (CL; 130 μmol photons m⁻² s⁻¹) conditions, followed by exposure to either CL or high light (HL; 1,100 μmol photons m⁻² s⁻¹) in ambient CO₂ for up to 6 h. Under both light conditions, ¹⁴C incorporation was strongly decreased for Chl a and moderately suppressed for β-C in Linco-treated leaves, showing a marked decline of PSII efficiency (F(v)/F(m)) and β-C content compared with water-treated leaves. Partial inhibition of carotenoid biosynthesis by NF caused no or only a minor decrease in F(v)/F(m) and Chl a turnover under both conditions, while the β-C content significantly declined and high ¹⁴C labeling was found for phytoene, the substrate of phytoene desaturase. Together, the results suggest coordinated turnover of Chl a and D1, but somewhat different regulation for β-C turnover, in Arabidopsis leaves. Inhibition of carotenoid biosynthesis by NF may initially enhance metabolic flux in the pathway upstream of phytoene, presumably compensating for short supply of β-C. Our observations are also in line with the notion that HL-induced accumulation of xanthophylls may involve a precursor pool which is distinct from that for β-C turnover.

Keyword(s): Arabidopsis: metabolism (MeSH) ; Carbon Dioxide: metabolism (MeSH) ; Carotenoids: metabolism (MeSH) ; Chlorophyll: biosynthesis (MeSH) ; Isotope Labeling (MeSH) ; Light (MeSH) ; Lincomycin: pharmacology (MeSH) ; Photosystem II Protein Complex: metabolism (MeSH) ; Plant Leaves: metabolism (MeSH) ; Pyridazines: pharmacology (MeSH) ; beta Carotene: biosynthesis (MeSH) ; Photosystem II Protein Complex ; Pyridazines ; Carbon Dioxide ; Chlorophyll ; Lincomycin ; norflurazone ; Carotenoids ; chlorophyll a ; phytoene ; beta Carotene ; J ; Arabidopsis thaliana (auto) ; beta-Carotene (auto) ; Chl (auto) ; Lincomycin (auto) ; Norflurazon (auto) ; Photoinhibition (auto)