Journal Article

FZJ-2015-04620

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Distinctive Glial and Neuronal Interfacing on Nanocrystalline Diamond

Bendali, A. (Corresponding Author) ; Agnès, C. ; Meffert, S.FZJ* ; Forster, V. ; Bongrain, A. ; Arnault, J.-C. ; Sahel, J.-A. ; Offenhäusser, A.FZJ* ; Bergonzo, P. ; Picaud, S. (Corresponding Author)

2014
PLoS Lawrence, Kan.

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Please use a persistent id in citations: http://hdl.handle.net/2128/8948  doi:10.1371/journal.pone.0092562

Abstract: Direct electrode/neuron interfacing is a key challenge to achieve high resolution of neuronal stimulation required for visual prostheses. Neuronal interfacing on biomaterials commonly requires the presence of glial cells and/or protein coating. Nanocrystalline diamond is a highly mechanically stable biomaterial with a remarkably large potential window for the electrical stimulation of tissues. Using adult retinal cell cultures from rats, we found that glial cells and retinal neurons grew equally well on glass and nanocrystalline diamond. The use of a protein coating increased cell survival, particularly for glial cells. However, bipolar neurons appeared to grow even in direct contact with bare diamond. We investigated whether the presence of glial cells contributed to this direct neuron/diamond interface, by using purified adult retinal ganglion cells to seed diamond and glass surfaces with and without protein coatings. Surprisingly, these fully differentiated spiking neurons survived better on nanocrystalline diamond without any protein coating. This greater survival was indicated by larger cell numbers and the presence of longer neurites. When a protein pattern was drawn on diamond, neurons did not grow preferentially on the coated area, by contrast to their behavior on a patterned glass. This study highlights the interesting biocompatibility properties of nanocrystalline diamond, allowing direct neuronal interfacing, whereas a protein coating was required for glial cell growth.

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Contributing Institute(s):

1. Bioelektronik (ICS-8)

Research Program(s):

1. 453 - Physics of the Cell (POF2-453) (POF2-453)

Appears in the scientific report 2015

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Database coverage:
; ; ; ; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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