Journal Article

FZJ-2015-05279

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A multiscale approach for the reconstruction of the fiber architecture of the human brain based on 3D-PLI

Reckfort, J. (Corresponding author)FZJ* ; Wiese, H. (Corresponding author)FZJ* ; Pietrzyk, U.FZJ* ; Zilles, K.FZJ* ; Amunts, K. (Corresponding author)FZJ* ; Axer, M.FZJ*

2015
Frontiers Research Foundation Lausanne

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Please use a persistent id in citations: http://hdl.handle.net/2128/9094  doi:10.3389/fnana.2015.00118

Abstract: Structural connectivity of the brain can be conceptionalized as a multiscale organization. The present study is built on 3D-Polarized Light Imaging (3D-PLI), a neuroimaging technique targeting the reconstruction of nerve fiber orientations and therefore contributing to the analysis of brain connectivity. Spatial orientations of the fibers are derived from birefringence measurements of unstained histological sections that are interpreted by means of a voxel-based analysis. This implies that a single fiber orientation vector is obtained for each voxel, which reflects the net effect of all comprised fibers. We have utilized two polarimetric setups providing an object space resolution of 1.3 μm/px (microscopic setup) and 64 μm/px (macroscopic setup) to carry out 3D-PLI and retrieve fiber orientations of the same tissue samples, but at complementary voxel sizes (i.e., scales). The present study identifies the main sources which cause a discrepancy of the measured fiber orientations observed when measuring the same sample with the two polarimetric systems. As such sources the differing optical resolutions and diverging retardances of the implemented waveplates were identified. A methodology was implemented that enables the compensation of measured different systems' responses to the same birefringent sample. This opens up new ways to conduct multiscale analysis in brains by means of 3D-PLI and to provide a reliable basis for the transition between different scales of the nerve fiber architecture.

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Contributing Institute(s):

1. Strukturelle und funktionelle Organisation des Gehirns (INM-1)

Research Program(s):

1. 571 - Connectivity and Activity (POF3-571) (POF3-571)
2. HBP - The Human Brain Project (604102) (604102)
3. SMHB - Supercomputing and Modelling for the Human Brain (HGF-SMHB-2013-2017) (HGF-SMHB-2013-2017)
4. NIH-R01MH092311 - Postnatal Development of Cortical Receptors and White Matter Tracts in the Vervet (NIH-R01MH092311) (NIH-R01MH092311)

Appears in the scientific report 2015

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Database coverage:
; ; ; BIOSIS Previews ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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