Journal Article

PreJuSER-21087

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Label-free electrical detection of DNA by means of field-effect nanoplate capacitors: Experiments and modeling

Abouzar, M. H.FZJ* ; Poghossian, A.FZJ* ; Cherstvy, A. G.FZJ* ; Pedraza, A. MN.FZJ* ; Ingebrandt, S.FZJ* ; Schöning, M. J.FZJ*

2012
Wiley-VCH Weinheim

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Please use a persistent id in citations: doi:10.1002/pssa.201100710

Abstract: Label-free electrical detection of consecutive deoxyribonucleic acid (DNA) hybridization/denaturation by means of an array of individually addressable field-effect-based nanoplate silicon-on-insulator (SOI) capacitors modified with gold nanoparticles (Au-NP) is investigated. The proposed device detects charge changes on Au-NP/DNA hybrids induced by the hybridization or denaturation event. DNA hybridization was performed in a high ionic-strength solution to provide a high hybridization efficiency. On the other hand, to reduce the screening of the DNA charge by counter ions and to achieve a high sensitivity, the sensor signal induced by the hybridization and denaturation events was measured in a low ionic-strength solution. High sensor signals of about 120, 90, and 80 mV were registered after the DNA hybridization, denaturation, and re-hybridization events, respectively. Fluorescence microscopy has been applied as reference method to verify the DNA immobilization, hybridization, and denaturation processes. An electrostatic charge-plane model for potential changes at the gate surface of a nanoplate field-effect sensor induced by the DNA hybridization has been developed taking into account both the Debye length and the distance of the DNA charge from the gate surface. (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keyword(s): J ; DNA (auto) ; field-effect (auto) ; gold nanoparticle (auto) ; label-free detection (auto) ; nanoplate capacitor (auto)

Note: The authors thank X. T. Vu and J. Gun for valuable discussions. A. G. Cherstvy gratefully acknowledges the financial support by the Deutsche Forschungsgemeinschaft (DFG Grant CH 707/5-1).

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Contributing Institute(s):

1. Theorie der weichen Materie und Biophysik (ICS-2)
2. Bioelektronik (PGI-8)
3. Jülich-Aachen Research Alliance - Fundamentals of Future Information Technology (JARA-FIT)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)
2. 423 - Sensorics and bioinspired systems (POF2-423) (POF2-423)

Appears in the scientific report 2012

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Database coverage:
; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection ; Zoological Record

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