Journal Article

PreJuSER-56130

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Changes in the level of poly(Phe) synthesis in Escherichia coli ribosomes containing mutants of L4 ribosomal protein from Thermus thermophilus can be explained by structural changes in the peptidyltransferase center: a molecular dynamics simulation anal.

Papadopoulos, G. ; Grudinin, S.FZJ* ; Kalpaxis, D. ; Choli-Papadopoulou, T.

2006
Springer Berlin

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Please use a persistent id in citations: doi:10.1007/s00249-006-0076-4

Abstract: Data from polyphenylalanine [poly(Phe)] synthesis determination in the presence and in the absence of erythromycin have been used in conjunction with Molecular Dynamics Simulation analysis, in order to localize the functional sites affected by mutations of Thermus thermophilus ribosomal protein L4 incorporated in Escherichia coli ribosomes. We observed that alterations in ribosome capability to synthesize poly(Phe) in the absence of erythromycin were mainly correlated to shifts of A2062 and C2612 of 23S rRNA, while in the presence of erythromycin they were correlated to shifts of A2060 and U2584 of 23S rRNA. Our results suggest a means of understanding the role of the extended loop of L4 ribosomal protein in ribosomal peptidyltransferase center.

Keyword(s): Amino Acid Sequence (MeSH) ; Anti-Bacterial Agents: pharmacology (MeSH) ; Erythromycin: pharmacology (MeSH) ; Escherichia coli: drug effects (MeSH) ; Escherichia coli: genetics (MeSH) ; Escherichia coli: metabolism (MeSH) ; Models, Molecular (MeSH) ; Molecular Sequence Data (MeSH) ; Mutation (MeSH) ; Nucleic Acid Conformation (MeSH) ; Peptides: metabolism (MeSH) ; Peptidyl Transferases: chemistry (MeSH) ; Peptidyl Transferases: metabolism (MeSH) ; Protein Conformation (MeSH) ; RNA, Bacterial: genetics (MeSH) ; RNA, Ribosomal, 23S: genetics (MeSH) ; Ribosomal Proteins: chemistry (MeSH) ; Ribosomal Proteins: genetics (MeSH) ; Ribosomal Proteins: metabolism (MeSH) ; Ribosomes: genetics (MeSH) ; Ribosomes: metabolism (MeSH) ; Thermus thermophilus: metabolism (MeSH) ; Anti-Bacterial Agents ; Peptides ; RNA, Bacterial ; RNA, Ribosomal, 23S ; Ribosomal Proteins ; ribosomal protein L4 ; Erythromycin ; polyphenylalanine ; Peptidyl Transferases ; J ; molecular dynamics (auto) ; ribosomal function (auto) ; erythromycin (auto)

Note: Record converted from VDB: 12.11.2012

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Research Program(s):

1. Kondensierte Materie (P54)

Appears in the scientific report 2006
Notes: Nachtrag

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