Journal Article

FZJ-2016-04139

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Slip of grip of a molecular motor on a crowded track: Modeling shift of reading frame of ribosome on RNA template

Mishra, B. (Corresponding author) ; Schütz, G. M.FZJ* ; Chowdhury, D.

2016
EDP Science65224 Les-Ulis

This record in other databases:    

Please use a persistent id in citations: http://hdl.handle.net/2128/22906  doi:10.1209/0295-5075/114/68005

Abstract: We develop a stochastic model for the programmed frameshift of ribosomes synthesizing a protein while moving along a mRNA template. Normally the reading frame of a ribosome decodes successive triplets of nucleotides on the mRNA in a step-by-step manner. We focus on the programmed shift of the ribosomal reading frame, forward or backward, by only one nucleotide which results in a fusion protein; it occurs when a ribosome temporarily loses its grip to its mRNA track. Special "slippery" sequences of nucleotides and also downstream secondary structures of the mRNA strand are believed to play key roles in programmed frameshift. Here we explore the role of an hitherto neglected parameter in regulating −1 programmed frameshift. Specifically, we demonstrate that the frameshift frequency can be strongly regulated also by the density of the ribosomes, all of which are engaged in simultaneous translation of the same mRNA, at and around the slippery sequence. Monte Carlo simulations support the analytical predictions obtained from a mean-field analysis of the stochastic dynamics

---

Contributing Institute(s):

1. Theorie der Weichen Materie und Biophysik (ICS-2)

Research Program(s):

1. 551 - Functional Macromolecules and Complexes (POF3-551) (POF3-551)

Appears in the scientific report 2016

---

Database coverage:
; ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; No Authors Fulltext ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

---