Journal Article

FZJ-2018-01952

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Tunable Ampere phase plate for low dose imaging of biomolecular complexes

Tavabi, A. H. (Corresponding author)FZJ* ; Beleggia, M. ; Migunov, V.FZJ* ; Savenko, A. ; Öktem, O. ; Dunin-Borkowski, R.FZJ* ; Pozzi, G.FZJ*

2018
Nature Publishing Group London

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Please use a persistent id in citations: http://hdl.handle.net/2128/18496  doi:10.1038/s41598-018-23100-3

Abstract: A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere’s law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.

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Contributing Institute(s):

1. Physik Nanoskaliger Systeme (ER-C-1)
2. Materialwissenschaft u. Werkstofftechnik (ER-C-2)

Research Program(s):

1. 143 - Controlling Configuration-Based Phenomena (POF3-143) (POF3-143)

Appears in the scientific report 2018

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