Journal Article

FZJ-2020-00059

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png An Ustilago maydis chassis for itaconic acid production without by‐products

Becker, J. ; Hosseinpour Tehrani, H. ; Gauert, M. ; Mampel, J. ; Blank, L. M. ; Wierckx, N. (Corresponding author)FZJ*

2020
Wiley-Blackwell Oxford

This record in other databases:      

Please use a persistent id in citations: http://hdl.handle.net/2128/24433  doi:10.1111/1751-7915.13525

Abstract: Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild‐type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by‐product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker‐free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by‐product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity.

Note: Biotechnologie 1

---

Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 581 - Biotechnology (POF3-581) (POF3-581)

Appears in the scientific report 2020

---

Database coverage:
; ; ; ; BIOSIS Previews ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Ebsco Academic Search ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---