Journal Article

FZJ-2020-00643

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Correlating Surface Plasmon Resonance Microscopy of Living and Fixated Cells with Electron Microscopy Allows for Investigation of Potential Preparation Artifacts

Kreysing, E. (Corresponding author)FZJ* ; Seyock, S. ; Hassani, H.FZJ* ; Brauweiler-Reuters, E.FZJ* ; Neumann, E.FZJ* ; Offenhäusser, A. (Corresponding author)FZJ*

2020
Wiley-VCH Weinheim

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Please use a persistent id in citations: http://hdl.handle.net/2128/25758  doi:10.1002/admi.201901991

Abstract: The investigation of the cell–substrate interface is of great importance for a broad spectrum of areas such as biomedical engineering, brain‐chip interfacing, and fundamental research. Due to its unique resolution and the prevalence of instruments, electron microscopy (EM) is used as one of the standard techniques for the analysis of the cell–substrate interface. However, possible artifacts that might be introduced by the required sample preparation have been the subject of speculation for decades. Due to recent advances in surface plasmon resonance microscopy (SPRM), the technique now offers a label‐free alternative for the interface characterization with nanometer resolution in axial direction. In contrast to EM, SPRM studies do not require fixation and can therefore be performed on living cells. Here, a workflow that allows for the quantification of the impact of chemical fixation on the cell–substrate interface is presented. These measurements confirm that chemical fixation preserves the average cell–substrate distances in the majority of studied cells. Furthermore, it is possible to correlate the SPRM measurements with EM images of the cell–substrate interface of the exact same cells, thus identifying regions of good agreement between the two methods and revealing artifacts introduced during further sample preparation.

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Contributing Institute(s):

1. Bioelektronik (ICS-8)
2. Helmholtz - Nanofacility (HNF)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2020

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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