Journal Article

FZJ-2020-03786

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Switch of fractal properties of DNA in chicken erythrocytes nuclei by mechanical stress

Grigoriev, S. V. (Corresponding author) ; Iashina, E. G. ; Bairamukov, V. Y. ; Pipich, V.FZJ* ; Radulescu, A.FZJ* ; Filatov, M. V. ; Pantina, R. A. ; Varfolomeeva, E. Y.

2020
Inst. Woodbury, NY

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Please use a persistent id in citations: http://hdl.handle.net/2128/25828  doi:10.1103/PhysRevE.102.032415

Abstract: The small-angle neutron scattering (SANS) on the chicken erythrocyte nuclei demonstrates the bifractal nature of the chromatin structural organization. Use of the contrast variation (D2O−H2O) in SANS measurements reveals the differences in the DNA and protein arrangements inside the chromatin substance. It is the DNA that serves as a framework that constitutes the bifractal behavior showing the mass fractal properties with D=2.22 at a smaller scale and the logarithmic fractal behavior with D≈3 at a larger scale. The protein spatial organization shows the mass fractal properties with D≈2.34 throughout the whole nucleus. The borderline between two fractal levels can be significantly shifted toward smaller scales by centrifugation of the nuclei disposed on the dry substrate, since nuclei suffer from mechanical stress transforming them to a disklike shape. The height of this disk measured by atomic force microscopy (AFM) coincides closely with the fractal borderline, thus characterizing two types of the chromatin with the soft (at larger scale) and rigid (at smaller scale) properties. The combined SANS and AFM measurements demonstrate the stress induced switch of the DNA fractal properties from the rigid, but loosely packed, mass fractal to the soft, but densely packed, logarithmic fractal.

Keyword(s): Polymers, Soft Nano Particles and Proteins (1st) ; Biology (2nd) ; Soft Condensed Matter (2nd) ; Medicine (2nd)

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Contributing Institute(s):

1. JCNS-FRM-II (JCNS-FRM-II)
2. Neutronenstreuung (JCNS-1)
3. Heinz Maier-Leibnitz Zentrum (MLZ)

Research Program(s):

1. 6G4 - Jülich Centre for Neutron Research (JCNS) (POF3-623) (POF3-623)
2. 6G15 - FRM II / MLZ (POF3-6G15) (POF3-6G15)

Experiment(s):

1. KWS-2: Small angle scattering diffractometer (NL3ao)
2. KWS-3: Very small angle scattering diffractometer with focusing mirror (NL3auS)

Appears in the scientific report 2020

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Electronics and Telecommunications Collection ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Web of Science Core Collection

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