Journal Article

FZJ-2021-03505

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png 18F-Labeled magnetic nanovectors for bimodal cellular imaging

Schütz, M. B. ; Renner, A. M. ; Ilyas, S. ; Lê, K. ; Guliyev, M. ; Krapf, P.FZJ* ; Neumaier, B.FZJ* ; Mathur, S. (Corresponding author)

2021
Royal Soc. of Chemistry Cambridge

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Please use a persistent id in citations: http://hdl.handle.net/2128/28673  doi:10.1039/D1BM00616A

Abstract: Surface modification of nanocarriers enables selective attachment to specific molecular targets within a complex biological environment. Besides the enhanced uptake due to specific interactions, the surfaceligands can be utilized for radiolabeling applications for bimodal imaging ensured by positron emission topography (PET) and magnetic resonance imaging (MRI) functions in one source. Herein, we describethe surface functionalization of magnetite (Fe3O4) with folic acid as a target vector. Additionally, the magnetic nanocarriers were conjugated with appropriate ligands for subsequent copper-catalyzed azide–alkyne cycloaddition or carbodiimide coupling reactions to successfully achieve radiolabeling with the PET-emitter 18F. The phase composition (XRD) and size analysis (TEM) confirmed the formation of Fe3O4nanoparticles (6.82 nm ± 0.52 nm). The quantification of various surface functionalities was performed by Fourier-transform infrared spectroscopy (FT-IR) and ultraviolet-visible microscopy (UV-Vis). An innovativemagnetic-HPLC method was developed in this work for the determination of the radiochemical yield of the 18F-labeled NPs. The as-prepared Fe3O4 particles demonstrated high radiochemical yields andshowed high cellular uptake in a folate receptor overexpressing MCF-7 cell line, validating bimodalimaging chemical design and a magnetic HPLC system. This novel approach, combining folic acidcappedFe3O4 nanocarriers as a targeting vector with 18F labeling, is promising to apply this probe forbimodal PET/MR-studies.

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Contributing Institute(s):

1. Nuklearchemie (INM-5)

Research Program(s):

1. 5253 - Neuroimaging (POF4-525) (POF4-525)

Appears in the scientific report 2021

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Database coverage:
; ; ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Essential Science Indicators ; IF >= 5 ; JCR ; National-Konsortium ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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