Journal Article

FZJ-2021-04232

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Microbial Polyphenol Production in a Biphasic Process

Tharmasothirajan, A.FZJ* ; Wellfonder, M. ; Marienhagen, J. (Corresponding author)FZJ*

2021
ACS Publ. Washington, DC

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Please use a persistent id in citations: http://hdl.handle.net/2128/31270  doi:10.1021/acssuschemeng.1c05865

Abstract: Microbial synthesis of aromatic compounds is generally limited by inherent product toxicity toward the producing cells. Here, in situ extractive strategies represent an efficient approach to avoid such toxic effects and to increase the overall process performance. We conducted a solvent screening to identify suitable organic solvents to develop a biphasic extractive strategy for microbial plant polyphenol production using Corynebacterium glutamicum. From 10 pre-selected organic solvents, tributyrin (TB) showed the best biocompatibility and was chosen for the biphasic extraction process due to its beneficial effect on partitioning and solubility of the plant polyphenol resveratrol. In bioreactors, biphasic cultivation with TB allowed for a product titer of 7.5 mM (1.71 g L–1) resveratrol with a volumetric productivity of 0.26 mM h–1 and a product yield of 0.92 mol mol–1. This biphasic cultivation procedure can be directly employed for the synthesis of other aromatics with similar properties using C. glutamicum.

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Contributing Institute(s):

1. Biotechnologie (IBG-1)

Research Program(s):

1. 2172 - Utilization of renewable carbon and energy sources and engineering of ecosystem functions (POF4-217) (POF4-217)

Appears in the scientific report 2022

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Engineering, Computing and Technology ; Current Contents - Physical, Chemical and Earth Sciences ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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