Journal Article

FZJ-2022-03076

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png A MademoiseLLE domain binding platform links the key RNA transporter to endosomes

Devan, S.-K. ; Schott-Verdugo, S.FZJ* ; Müntjes, K. ; Bismar, L. ; Reiners, J. ; Hachani, E. ; Schmitt, L. ; Höppner, A. ; Smits, S. H. (Corresponding author) ; Gohlke, H. (Corresponding author)FZJ* ; Feldbrügge, M. (Corresponding author)

2022
Public Library of Science San Francisco, Calif.

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Please use a persistent id in citations: http://hdl.handle.net/2128/31670  doi:10.1371/journal.pgen.1010269

Abstract: Spatiotemporal expression can be achieved by transport and translation of mRNAs at defined subcellular sites. An emerging mechanism mediating mRNA trafficking is microtubule-dependent co-transport on shuttling endosomes. Although progress has been made in identifying various components of the endosomal mRNA transport machinery, a mechanistic understanding of how these RNA-binding proteins are connected to endosomes is still lacking. Here, we demonstrate that a flexible MademoiseLLE (MLLE) domain platform within RNA-binding protein Rrm4 of Ustilago maydis is crucial for endosomal attachment. Our structure/function analysis uncovered three MLLE domains at the C-terminus of Rrm4 with a functionally defined hierarchy. MLLE3 recognises two PAM2-like sequences of the adaptor protein Upa1 and is essential for endosomal shuttling of Rrm4. MLLE1 and MLLE2 are most likely accessory domains exhibiting a variable binding mode for interaction with currently unknown partners. Thus, endosomal attachment of the mRNA transporter is orchestrated by a sophisticated MLLE domain binding platform.

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Contributing Institute(s):

1. Jülich Supercomputing Center (JSC)
2. John von Neumann - Institut für Computing (NIC)
3. Strukturbiochemie (IBI-7)
4. Bioinformatik (IBG-4)

Research Program(s):

1. 5111 - Domain-Specific Simulation & Data Life Cycle Labs (SDLs) and Research Groups (POF4-511) (POF4-511)
2. 2171 - Biological and environmental resources for sustainable use (POF4-217) (POF4-217)
3. Forschergruppe Gohlke (hkf7_20200501) (hkf7_20200501)
4. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2022

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