Contribution to a conference proceedings/Contribution to a book

FZJ-2023-00150

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Effect of membrane deformation on electrical firing in rat cortical neurons during electrophysiological measurements

Cepkenovic, B. (First author)FZJ* ; Maybeck, V.FZJ* ; Offenhäusser, A. (Corresponding author)FZJ*

2021

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Please use a persistent id in citations: http://hdl.handle.net/2128/34123  doi:10.1007/s12551-021-00845-2

Abstract: INTRODUCTION From patch-clamp to 3D nanoelectrodes, tight mechanical coupling with the neuronal membrane is essential to secure the high amplitude electrical recording. As a byproduct, both approaches induce membrane deformation. In the former, the membrane is acutely deformed by the glass pipette,while in the latter, the membrane spontaneously engulfs the 3D vertical nanostructure. OBJECTIVES In line with the discoveries pointing to the existence of mechanosensitive ion channels in neurons, we combined electrophysiology with functional imaging to test the effect of acute and chronic membrane deformation on rat cortical neurons’ electrical properties and firing dynamics.MATERIALS AND METHODS To estimate the effect of patch-clamp induced acute deformation, we combined semi-blind patch-clamp with calcium-imaging. Additionally,we utilized patch-clamp to investigate whether the long-term exposure to vertical topology on 3D nanoelectrodes influences the neurons’ electrical properties. All measurements were performed on rat cortical neurons starting from 2 weeks in culture.DISCUSSION AND RESULTS Calcium-imaging measurements during the formation of giga-seal have demonstrated that patch-clamp targeted neurons respond to the mechanical perturbation with plateau-shaped calcium signals (N = 29). Moreover,up to 100% of neurons in 0.185 mm 2 area responded in a similar trend.This finding suggests that acute deformation affects not only the targeted neuron, but also the immediate network. Furthermore, the comparison of neurons on flat surface and neurons on 3D nanoelectrodes showed no statistically significant difference in excitability and action potential firing.CONCLUSION Overall, these results recognize the effects of acute, patch-clamp mediated mechanical perturbation on the targeted neuron, as well as the immediate network. On the other hand, no changes were present with chronic membrane deformations during the spontaneous engulfment of vertical nanostructures. Keywords: electrophysiology, membrane, neurons

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5244 - Information Processing in Neuronal Networks (POF4-524) (POF4-524)

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