Association of Protein Distribution and GeneExpression Revealed by PET and Post-MortemQuantification in the Serotonergic System of theHuman Brain

Komorowski, A.; James, G. M.; Hienert, M.; Wadsak, W.; Hahn, A.; Vanicek, T.; Bauer, Andreas; Traub-Weidinger, T.; Spies, M.; Hacker, M.; Gryglewski, G.; Mitterhauser, M.; Lanzenberger, R.; Kasper, S.; Kautzky, A.; Winkler, D.; Phillipe, C.

doi:10.1093/cercor/bhw355

Journal Article

FZJ-2017-05296

Association of Protein Distribution and GeneExpression Revealed by PET and Post-MortemQuantification in the Serotonergic System of theHuman Brain

Komorowski, A. ; James, G. M. ; Phillipe, C. ; Gryglewski, G. ; Bauer, A.FZJ* ; Hienert, M. ; Spies, M. ; Kautzky, A. ; Vanicek, T. ; Hahn, A. ; Traub-Weidinger, T. ; Winkler, D. ; Wadsak, W. ; Mitterhauser, M. ; Hacker, M. ; Kasper, S. ; Lanzenberger, R. (Corresponding author)

2017
Oxford Univ. Press Oxford

Cerebral cortex 27, 117-130 (2017) [10.1093/cercor/bhw355]

This record in other databases:

Please use a persistent id in citations: http://hdl.handle.net/2128/14969 doi:10.1093/cercor/bhw355

Abstract: Regional differences in posttranscriptional mechanisms may influence in vivo protein densities. The association of positron emission tomography (PET) imaging data from 112 healthy controls and gene expression values from the Allen Human Brain Atlas, based on post-mortem brains, was investigated for key serotonergic proteins. PET binding values and gene expression intensities were correlated for the main inhibitory (5-HT1A) and excitatory (5-HT2A) serotonin receptor, the serotonin transporter (SERT) as well as monoamine oxidase-A (MAO-A), using Spearman's correlation coefficients (rs) in a voxel-wise and region-wise analysis. Correlations indicated a strong linear relationship between gene and protein expression for both the 5-HT1A (voxel-wise rs = 0.71; region-wise rs = 0.93) and the 5-HT2A receptor (rs = 0.66; 0.75), but only a weak association for MAO-A (rs = 0.26; 0.66) and no clear correlation for SERT (rs = 0.17; 0.29). Additionally, region-wise correlations were performed using mRNA expression from the HBT, yielding comparable results (5-HT1Ars = 0.82; 5-HT2Ars = 0.88; MAO-A rs = 0.50; SERT rs = −0.01). The SERT and MAO-A appear to be regulated in a region-specific manner across the whole brain. In contrast, the serotonin-1A and -2A receptors are presumably targeted by common posttranscriptional processes similar in all brain areas suggesting the applicability of mRNA expression as surrogate parameter for density of these proteins.

Classification: