Journal Article

FZJ-2019-03877

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells

Kolašinac, R.FZJ* ; Jaksch, S.FZJ* ; Dreissen, G.FZJ* ; Braeutigam, A. ; Merkel, R.FZJ* ; Csiszar, A. (Corresponding author)FZJ*

2019
MDPI Basel

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Please use a persistent id in citations: http://hdl.handle.net/2128/22498  doi:10.3390/nano9071025

Abstract: Lipid-based nanoparticles, also called vesicles or liposomes, can be used as carriers for drugs or many types of biological macromolecules, including DNA and proteins. Efficiency and speed of cargo delivery are especially high for carrier vesicles that fuse with the cellular plasma membrane. This occurs for lipid mixture containing equal amounts of the cationic lipid DOTAP and a neutral lipid with an additional few percents of an aromatic substance. The fusion ability of such particles depends on lipid composition with phosphoethanolamine (PE) lipids favoring fusion and phosphatidyl-choline (PC) lipids endocytosis. Here, we examined the effects of temperature, ionic strength, osmolality, and pH on fusion efficiency of cationic liposomes with Chinese hamster ovary (CHO) cells. The phase state of liposomes was analyzed by small angle neutron scattering (SANS). Our results showed that PC containing lipid membranes were organized in the lamellar phase. Here, fusion efficiency depended on buffer conditions and remained vanishingly small at physiological conditions. In contrast, SANS indicated the coexistence of very small (~50 nm) objects with larger, most likely lamellar structures for PE containing lipid particles. The fusion of such particles to cell membranes occurred with very high efficiency at all buffer conditions. We hypothesize that the altered phase state resulted in a highly reduced energetic barrier against fusion

Keyword(s): Health and Life (1st) ; Medicine (2nd) ; Soft Condensed Matter (2nd)

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Contributing Institute(s):

1. Biomechanik (ICS-7)
2. JCNS-FRM-II (JCNS-FRM-II)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Experiment(s):

1. KWS-2: Small angle scattering diffractometer (NL3ao)

Appears in the scientific report 2019

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Database coverage:
; ; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; IF < 5 ; JCR ; PubMed Central ; Science Citation Index Expanded ; Web of Science Core Collection

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