Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation

Höfig, Henning; Katranidis, Alexandros; Fitter, Jörg; Kempe, Daryan; Yukhnovets, Olessya; Remes, Cristina; Kempf, Noemie

doi:10.1038/s42003-019-0709-7

Journal Article

FZJ-2019-05903

Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation

Höfig, H. ; Yukhnovets, O.FZJ* ; Remes, C. ; Kempf, N. ; Katranidis, A.FZJ* ; Kempe, D. ; Fitter, J. (Corresponding author)FZJ*

2019
Springer Nature London

Communications biology 2(1), 459 (2019) [10.1038/s42003-019-0709-7]

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Please use a persistent id in citations: http://hdl.handle.net/2128/23599 doi:10.1038/s42003-019-0709-7

Abstract: Life on the molecular scale is based on a complex interplay of biomolecules under which the ability of binding is crucial. Fluorescence based two-color coincidence detection (TCCD) is commonly used to characterize molecular binding, but suffers from an underestimation of coincident events. Here, we introduce a brightness-gated TCCD which overcomes this limitation and benchmark our approach with two custom-made calibration samples. Applied to a cell-free protein synthesis assay, brightness-gated TCCD unraveled a previously disregarded mode of translation initiation in bacteria.

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