Home > Publications database > Trimmomatic: a flexible trimmer for Illumina sequence data |
Journal Article | FZJ-2016-00423 |
; ;
2014
Oxford Univ. Press
Oxford
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Please use a persistent id in citations: http://hdl.handle.net/2128/9693 doi:10.1093/bioinformatics/btu170
Abstract: Motivation: Although many next-generation sequencing (NGS) read preprocessing tools already existed, we could not find any tool or combination of tools that met our requirements in terms of flexibility, correct handling of paired-end data and high performance. We have developed Trimmomatic as a more flexible and efficient preprocessing tool, which could correctly handle paired-end data.Results: The value of NGS read preprocessing is demonstrated for both reference-based and reference-free tasks. Trimmomatic is shown to produce output that is at least competitive with, and in many cases superior to, that produced by other tools, in all scenarios tested.
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