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| Hauptseite > Publikationsdatenbank > Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion |
| Hauptseite > Publikationsdatenbank > Miniaturized planar lipid bilayer: increased stability, low electric noise and fast fluid perfusion |
| Journal Article | PreJuSER-61424 |
; ; ; ; ;
2008
Springer
Berlin
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Please use a persistent id in citations: doi:10.1007/s00216-007-1647-7
Abstract: A microfluidic device was designed allowing the formation of a planar lipid bilayer across a micron-sized aperture in a glass slide sandwiched between two polydimethylsiloxane channel systems. By flushing giant unilamellar vesicles through a 500-microm-wide channel above the hole, we were able to form a planar lipid bilayer across the hole, resulting in a giga-seal. We demonstrate incorporation of biological nanopores into the bilayer. This miniaturized system offers noise recordings comparable to open head-stage noise (under 1 pA RMS at 10 kHz), fast precision perfusion on each side of the membrane and the use of nanoliter analyte volumes. This technique shows a promising potential for automation and parallelization of electrophysiological setups.
Keyword(s): Cytoplasm: metabolism (MeSH) ; Dimethylpolysiloxanes: chemistry (MeSH) ; Electrochemistry: methods (MeSH) ; Electrophysiology: instrumentation (MeSH) ; Electrophysiology: methods (MeSH) ; Equipment Design (MeSH) ; Ions (MeSH) ; Lipid Bilayers: chemistry (MeSH) ; Liposomes: chemistry (MeSH) ; Microfluidic Analytical Techniques (MeSH) ; Miniaturization (MeSH) ; Nanotechnology: methods (MeSH) ; Patch-Clamp Techniques (MeSH) ; Perfusion (MeSH) ; Silicones: chemistry (MeSH) ; Solvents: chemistry (MeSH) ; Dimethylpolysiloxanes ; Ions ; Lipid Bilayers ; Liposomes ; Silicones ; Solvents ; baysilon ; J ; microfluidics (auto) ; planar bilayer (auto) ; electrophysiology (auto) ; membrane channel (auto)
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