Journal Article

FZJ-2020-01007

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Spatial filter and its application in three-dimensional single molecule localization microscopy

Fan, X. (Corresponding author) ; Hendriks, J. ; Comini, M. ; Katranidis, A.FZJ* ; Büldt, G.FZJ* ; Gensch, T. (Corresponding author)FZJ*

2020
IOP Publ. Bristol

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Please use a persistent id in citations: http://hdl.handle.net/2128/24640  doi:10.1088/2050-6120/ab7e0f

Abstract: Single molecule localization microscopy (SMLM) allows the imaging of cellular structures with resolutions five to ten times below the diffraction limit of optical microscopy. It was originally introduced as a two-dimensional technique based on the localization of single emitters as projection onto the x-y imaging plane. The determination of the axial position of a fluorescent emitter is only possible by additional information. Here we report a method (spatial filter SMLM (SFSMLM)) that allows to determine the axial positions of fluorescent molecules and nanoparticles on the nanometer scale by the usage of two spatial filters, which are placed in two otherwise identical emission detection channels. SFSMLM allows axial localization in a range of ca. 1.5 μm with a localization precision of 15 - 30 nm in axial direction. The technique was utilized for localizing and imaging small cellular structures – e.g. actin filaments, vesicles and mitochondria - in three dimensions.

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Contributing Institute(s):

1. Zelluläre Biophysik (ICS-4)
2. Molekulare Biophysik (ICS-5)

Research Program(s):

1. 552 - Engineering Cell Function (POF3-552) (POF3-552)

Appears in the scientific report 2020

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; IF < 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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