Journal Article

FZJ-2021-03106

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png 18 O Isotope Labeling Combined with 31 P Nuclear Magnetic Resonance Spectroscopy for Accurate Quantification of Hydrolyzable Phosphorus Species in Environmental Samples

Wang, L. (First author)FZJ* ; Amelung, W.FZJ* ; Willbold, S. (Corresponding author)FZJ*

2021
American Chemical Society Columbus, Ohio

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Please use a persistent id in citations: http://hdl.handle.net/2128/28388  doi:10.1021/acs.analchem.0c03379

Abstract: 31P nuclear magnetic resonance (NMR) spectra can be biased due to the hydrolysis of labile P species during sample treatment and NMR analysis. This paper offers an approach to circumvent this problem by performing sample preparation and analysis in 18O-enriched medium. Heavy 18O isotope atoms were introduced into the resulting artificial hydrolysis products. The NMR signal of 18O-labeled P was shifted upfield relative to the unlabeled P nuclei in natural metabolites. This isotope shift enabled an immediate differentiation of artificial hydrolysis products from natural metabolites. Moreover, the hydrolysis products could be accurately quantified. Our data suggest that the extent to which artificial hydrolysis alters NMR spectra varies among different types of environmental samples. For instance, 72–84% of the detected monoesters in the organic soils of this study were actually artificially hydrolyzed diesters. By contrast, artificial hydrolysis products in the mineral soils used for this study accounted for less than 6% of the total monoesters. Polyphosphate was also hydrolyzed to yield 18O-labeled products in algal biomass.

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Contributing Institute(s):

1. Analytik (ZEA-3)

Research Program(s):

1. 2151 - Terrestrial ecosystems of the future (POF4-215) (POF4-215)

Appears in the scientific report 2021

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Database coverage:
; ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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