Journal Article

FZJ-2021-05923

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Randomly positioned gold nanoparticles as fluorescence enhancers in apta-immunosensor for malaria test

Minopoli, A. ; Della Ventura, B. ; Campanile, R. ; Tanner, J. A. ; Offenhäusser, A.FZJ* ; Mayer, D. (Corresponding author)FZJ* ; Velotta, R.Extern*

2021
Springer Wien [u.a.]

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Please use a persistent id in citations: http://hdl.handle.net/2128/33175  doi:10.1007/s00604-021-04746-9

Abstract: A plasmon-enhanced fluorescence-based antibody-aptamer biosensor — consisting of gold nanoparticles randomly immobilized onto a glass substrate via electrostatic self-assembly — is described for specific detection of proteins in whole blood. Analyte recognition is realized through a sandwich scheme with a capture bioreceptor layer of antibodies — covalently immobilized onto the gold nanoparticle surface in upright orientation and close-packed configuration by photochemical immobilization technique (PIT) — and a top bioreceptor layer of fluorescently labelled aptamers. Such a sandwich configuration warrants not only extremely high specificity, but also an ideal fluorophore-nanostructure distance (approximately 10–15 nm) for achieving strong fluorescence amplification. For a specific application, we tested the biosensor performance in a case study for the detection of malaria-related marker Plasmodium falciparum lactate dehydrogenase (PfLDH). The proposed biosensor can specifically detect PfLDH in spiked whole blood down to 10 pM (0.3 ng/mL) without any sample pretreatment. The combination of simple and scalable fabrication, potentially high-throughput analysis, and excellent sensing performance provides a new approach to biosensing with significant advantages compared to conventional fluorescence immunoassays

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Contributing Institute(s):

1. Bioelektronik (IBI-3)

Research Program(s):

1. 5241 - Molecular Information Processing in Cellular Systems (POF4-524) (POF4-524)

Appears in the scientific report 2022

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Database coverage:
; ; ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DEAL Springer ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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