Journal Article

PreJuSER-21271

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Quantitative Mapping of Averaged Focal Adhesion Dynamics in Migrating Cells by Shape Normalization

Möhl, C.FZJ* ; Kirchgeßner, N.FZJ* ; Schäfer, C.FZJ* ; Hoffmann, B.FZJ* ; Merkel, R.FZJ*

2012
Company of Biologists Limited Cambridge

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Please use a persistent id in citations: http://hdl.handle.net/2128/22882  doi:10.1242/jcs.090746

Abstract: The spatially ordered formation and disassembly of focal adhesions is a basic requirement for effective cell locomotion. Because focal adhesions couple the contractile actin-myosin network to the substrate, their distribution determines the pattern of traction forces propelling the cell in a certain direction. In the present study, we quantitatively analyzed the spatial patterning of cell-substrate adhesion in migrating cells by mapping averaged focal adhesion growth dynamics to a standardized cell coordinate system. These maps revealed distinct zones of focal adhesion assembly, disassembly and stability and were strongly interrelated with corresponding actin flow and traction force patterns. Moreover, the mapping technique enables precise detection of even minute responses of adhesion dynamics upon targeted signaling perturbations. For example, the partial inhibition of vinculin phosphorylation was followed by the reduced number of newly formed adhesions, whereas growth dynamics of existing adhesions remained unaffected.

Keyword(s): J ; Actin flow (auto) ; Cell migration (auto) ; Focal adhesion dynamics (auto) ; Traction force (auto)

Note: This work was supported by the Bundesministerium fur Bildung und Forschung (BMBF) program [grant number 0315501].

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Contributing Institute(s):

1. Biomechanik (ICS-7)

Research Program(s):

1. BioSoft: Makromolekulare Systeme und biologische Informationsverarbeitung (P45)

Appears in the scientific report 2012

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Database coverage:
; ; BIOSIS Previews ; Current Contents - Life Sciences ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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